Oligonucleotides having selectably cleavable and/or abasic sites

Details Oligonucleotides having selectably cleavable and/or abasic sites Oligonucleotides having selectably cleavable and/or abasic sites

1990-07-27

1995-07-04

Robinson, Douglas W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2431, 536 2432, C12Q 108, C07H 2100

Patent

active

054301360

ABSTRACT:
Polynucleotides containing abasic, cleavable sites are provided. These polynucleotides are useful in a variety of biochemical and chemical contexts, particularly in solid phase nucleic acid hybridization assays because a captured probe can be released from the support. The polynucleotides have the structure ##STR1## where R is selected from the group consisting of 2-nitrobenzyl, 4-penten-1-yl, ##STR2## where R', R.sub.i and R.sub.j are as defined herein. One of the preferred embodiments is a polynucleotide where R is ##STR3##

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