Enzyme immobilization with an immobilizing reagent in vapor phas

Details Enzyme immobilization with an immobilizing reagent in vapor phas Enzyme immobilization with an immobilizing reagent in vapor phas

1982-03-16

1984-02-28

Nafe, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

435176, 435181, 435288, C12N 1100, C12N 1114, C12N 1106, C12M 140

Patent

active

044342292

ABSTRACT:
Immobilization of enzymes is carried out by covering the surface of a solid support with an enzyme and then contacting the enzyme with an immobilizing reagent in the vapor phase. The immobilizing reagent is preferably an aldehyde or a polymerized aldehyde. By having the immobilizing reagent in vapor phase, the immobilizing reagent concentration can be easily controlled by the vapor pressure. Additionally, there is obtained a uniform covering of immobilized enzyme on the support, and support surfaces which are uneven or curved can be covered with immobilized enzyme regardless of size.

REFERENCES:
patent: 3705084 (1972-12-01), Reynolds
patent: 4004979 (1977-01-01), Avrameas et al.
patent: 4240889 (1980-12-01), Yoda et al.
patent: 4251631 (1981-02-01), Simon

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