Methods and compositions for isolation and growth of kidney tubu

Details Methods and compositions for isolation and growth of kidney tubu Methods and compositions for isolation and growth of kidney tubu

1992-03-02

1995-07-04

Warden, Jill

Chemistry: molecular biology and microbiology

Spore forming or isolating process

530350, 530399, 514 2, A61K 3818

Patent

active

054299381

ABSTRACT:
Methods, including culture media conditions, which provide for isolation and purification of renal tubule stem cells and for in vitro kidney tubulogenesis are disclosed. The methods rely on culturing adult kidney cells in a culture media treated with combinations of transforming growth factor-.beta..sub.1, epidermal growth factor, and all-trans retinoic acid.

REFERENCES:
Vasios, Proc. Natl Acad Sci 86 9099-9103 (1989).
Taub, Proc Natl Acad Sci 87 4002-4006, 1990.
Humes Laboratory Investigation 64, 538-545 1991.
Montesano Cell 67, 901-908, 1991.

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