Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1993-09-23
1995-07-04
Gorgos, Kathryn
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, G01N 27447
Patent
active
054297281
ABSTRACT:
A separation capillary in a capillary electrophoresis apparatus is less than 20 microns high to restrict the flow velocity profile of the EOF to being substantially parabolic. The capillary is preferably rectangular with an aspect ratio of at least 2:1, and preferably at least 10:1, in order to increase bulk flow. Hydrostatic back pressure is applied to the capillary to reduce or cancel the EOF. The back pressure is preferably at least 50% as strong as the forward-directed EOF in order to reduce EOF, and thereby to increase resolution.
REFERENCES:
patent: 3705845 (1972-12-01), Everaerts
patent: 3869365 (1975-03-01), Sunden
Wim Th. Kok "Off-Column Detection with Pressure Compensation in Capilly Electrophoresis" Analytical Chemistry, vol. 65, No. 14 (Jul. 1993) 1853-1860.
Douglass McManigill et al "Dispersion in capillary electrophoresis with external flow control methods" Journal of Chromatography A vol. 652 No. 1 (Oct. 1993) 283-289.
U. R. Tjaden et al "Automated isotachophoretic analyte focusing for capillary zone electrophoresis in a single capillary using hydrodynamic back-pressure programming" Journal of Chromatography (Jul. 1993) 155-162.
Ranjit R. Deshmukh & Milan Bier "Counterflow in isotachophoresis: Computer simulation and experimental studies" Electrophoresis vol. 14 No. 3 (Mar. 1993) 205-213.
F. M. Everaerts, "Isotachophoretic Experiments with Counter Flow of Electrolyte" Journal of Chromatography 123 no month available (1976) 139-148.
Electrokinetic Dispersion in Capillary Electrophoresis, Ravindra Datta and Veerabhadra Kotamarthi, AIChE Journal, vol. 36, No. 6, pp. 916-923, Jun. 1990.
Isotachophoretic Experiments with a Counter Flow Electrolyte, F. M. Everarts, Th. P. E. M. Verheggen, and J. L. M. Van De Veene, 123 Journal of Chromatography, pp. 139-148, no month available 1976.
Gorgos Kathryn
Hewlett--Packard Company
Starsiak Jr. John S.
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