Portable apparatus and method for detection of methylxanthine ch

Details Portable apparatus and method for detection of methylxanthine ch Portable apparatus and method for detection of methylxanthine ch

1995-06-07

1998-10-06

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 19, 435 21, 435174, 435182, 436164, 436103, 436105, 422 55, 422 56, 422 57, C12Q 100

Patent

active

058174540

ABSTRACT:
A portable apparatus for detecting the presence of at least one methylxanthine chemical species such as caffeine or theophylline in a beverage comprises a first portion comprising an effective concentration of phosphodiesterase enzyme, a second portion comprising cyclic AMP, and means for indicating inhibition of degradation of the cyclic AMP by the phosphodiesterase due to the presence of the methylxanthine species. A method for determining the presence of at least one methylxanthine chemical species in a beverage comprises contacting at least one test portion of the beverage with effective concentrations of at least one phosphodiesterase enzyme and cyclic AMP, and further contacting the test portion with means for indicating the inhibition of the degradation of the cyclic AMP by the phosphodiesterase due to the presence of the methylxanthine species. The apparatus and method are advantageous in that they provide a simple and effective means of determining whether methylxanthine chemical species such as caffeine or theophylline are present in coffee, tea and other beverages.

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