Method of detecting mastitis in cows

Details Method of detecting mastitis in cows Method of detecting mastitis in cows

1984-06-13

1987-04-21

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 23, G01N 3353, C12Q 138

Patent

active

046596569

ABSTRACT:
There is disclosed a method for the quantitative determination of mastitis in a cow. This method comprises decaseinating and delipidating a milk sample from the cow to obtain a clear solution, substantially removing .alpha..sub.2 -macroglobulin from the sample, mixing the clear and substantially .alpha..sub.2 -macroglobulin free sample with a known amount to trypsin, and measuring the activity of the amount of trypsin which is in excess of the amount which is inactivated by the milk's antitrypsin.

REFERENCES:
patent: 3926732 (1975-12-01), Rosen et al.
patent: 4425330 (1984-01-01), Norcross et al.
de Rham et al., J. Dairy Res. (1982) 49, 587-596.
Honkanen-Buzalski et al., J. Dairy Res. (1981) 48, 213-23.
Honkanen-Buzalski et al., Chemical Abstracts, 96 (1982) #140648z.
Sandholm et al., Chemical Abstracts 100 (1984) #47513t.

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