Specific DNA probes in diagnostic microbiology

Details Specific DNA probes in diagnostic microbiology Specific DNA probes in diagnostic microbiology

1980-12-08

1982-11-09

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

23230B, 424 2, 424 8, 424 12, 424 13, 435 6, 435 34, 435 35, 435 36, 435 37, 435 38, C12Q 170

Patent

active

043585353

ABSTRACT:
Method and compositions for infectious disease diagnosis and epidemiology involving labeled nucleotide probes complementary to nucleic acid coding for a characteristic pathogen product. Clinical isolates are cultivated, expanding the number of microorganisms, the resulting colonies lysed, the genome normally denatured and then fixed. Alternatively, clinical samples (stool, sputum, pus, etc.) are spotted onto an inert support. The sample is treated in such a way that the DNA is liberated from microbes present in the sample and complexed onto the support. The DNA is normally denatured and fixed in this process. Subsequently, a labelled polynucleotide probe specific for a DNA sequence characteristic of a pathogenic product suspected of being present in the clinical sample is contacted with the fixed genomic single stranded nucleic acid under hybridizing conditions. Hybridization of probes to the single stranded nucleic acid is diagnostic of the presence of the pathogen.

REFERENCES:
patent: 3755086 (1973-08-01), Heimer
patent: 3930956 (1976-01-01), Juni
patent: 4038143 (1977-07-01), Juni
patent: 4275149 (1981-06-01), Litman et al.

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