Method of purifying putrescine n-methyltransferase from tobacco

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435815, C12N 910

Patent

active

053690230

ABSTRACT:
There is provided highly purified tobacco putrescine N-methyltransferase, a process for its purification, and production of PMT DNA sequence. The purification process includes the step of applying a tobacco root extract to an anion exchange medium and specifically eluting putrescine N-methyltransferase with an elution buffer comprising putrescine.

REFERENCES:
patent: 4594319 (1986-06-01), Sharma
patent: 4801540 (1989-01-01), Hiatt
patent: 5260205 (1993-11-01), Nakatami et al.
Bush and Saunders, "Physiological Aspects of Genetic Variation In Nicotine Content In Tobacco (Nicotiana tabacum)", Tobacco Abstracts, 23, p. 380 (1979).
Bush and Saunders, "Nicotine Biosynthetic Enzymes of Burley Tobacco", Tobacco Abstracts, 24, p. 260 (1980).
Chung and Blume, "Identification Of Nicotine Biosynthetic Intermediates In Tobacco Roots By Liquid Chromatography-Mass Spectrometry", J. of Chromatography, 474, pp. 329-333 (1989).
Cornelissen and Vandewiele, "Both RNA Level And Translation Efficiency Are Reduced By Anti-Sense RNA In Transgenic Tobacco", Nucleic Acids Res., 17, 3, pp. 833-843 (1989).
Cuozzo et al., "Viral Protection In Transgenic Tobacco Plants Expressing The Cucumber Mosaic Virus Coat Protein Or Its Antisense RNA", Biotechnology, 6, pp. 549-557 (1988).
Davies et al., "Quinoprotein Characteristics Of Methylputrescine Oxidase From Tobacco Roots", Phytochemistry, 28, 6, pp. 1573-1578 (1989).
Delauney et al., "A Stable Bifunctional Antisense Transcript Inhibiting Gene Expression In Transgenic Plants", Proc. Natl. Acad. Sci. USA, 85, pp. 4300-4304 (1988).
Ecker and Davis, "Inhibition Of Gene Expression In Plant Cells By Expression Of Antisense RNA", Proc. Natl. Acad. Sci. USA, 83, pp. 5372-5376 (1986).
Feth et al., "Determination Of Putrescine N-Methyltransferase By High Performance Liquid Chromatography", Phytochemistry, 24, pp. 921-923 (1985).
Feth et al., "Regulation In Tobacco Callus Of Enzyme Activities Of The Nicotine Pathway", Planta, 168, pp. 402-407 (1986).
Feth and Wagner, "Determination Of Ornithine, Putrescine, N-Methylputrescine And N-Methylpyroline Pools In Tobacco Tissue By High-Performance Liquid Chromatogrpahy", Physiologica Plantarum, 75, pp. 71-74 (1989).
Hashimoto et al., "Putrescine And Putrescine N-Methyltransferase In The Biosynthesis Of Tropane Alkaloids In Cultured Roots Of Hyoscyamus albus. I Biochemical Studies", Planta, 178, pp. 123-130 (1989).
Hashimoto et al., "Putrescine And Putrescine N-Methyltransferase In The Biosynthesis Of Tropane Alkaloids In Cultured Roots Of Hyoscyamus albus. II Incorporation Of Labeled Precursors", Planta, 178, pp. 131-137 (1989).
Hemenway et al., "Analysis Of The Mechanism Of Protection In Transgenic Plants Expressing The Potato Virus X Coat Protein Or Its Antisense RNA", EMBO J., 7, pp. 1273-1280.
Lichtenstein, "Anti-sense RNA As A Tool To Study Plant Gene Expression", Nature, 333, 801-802 (1988).
Mizusaki et al., "Phytochemical Studies On Tobacco Alkaloids XIV. The Occurrence And Properties Of Putrescine N-Methyltransferase In Tobacco Roots", Plant & Cell Physiol. 12, pp. 633-640 (1971).
Mizusaki et al., "Changes In The Activities Of Ornithine Decarboxylase, Putrescine N-Methyltransferase And N-Methylputrescine Oxidase In Tobacco Roots In Relation To Nicotine Biosynthesis", Plant and Cell Physiol., 14, pp.103-110 (1973).
Ohta and Yatazawa, "Metabolic Key Step Discriminating Nicotine Producing Tobacco Callus Strain From Ineffective One", Biochem. Physiol. Pflanzen, 175, pp. 382-385 (1980).
Rezanian et al., "Anti-Sense RNA's Of Cucumber Mosaic Virus In Transgenic Plants Assessed For Control Of The Virus", Plant Molecular Biology, 11, pp. 463-471 (1988).
Rodermel et al., "Nuclear-Organelle Interactions: Nuclear Antisense Gene Inhibits Ribulose Bisphosphate Carboxylase Enzyme Levels In Transformed Tobacco Plants", Cell, 55, pp. 673-681 (1988).
Rothstein et al., "Stable And Heritable Inhibition Of The Expression Of Nopaline Synthase In Tobacco Expressing Antisense RNA", Proc. Natl. Sci. USA, 84, pp. 8439-8443 (1987).
Sandler et al., "Inhibition Of Gene Expression In Transformed Plants By Antisense RNA", Plant Molecular Biology, 11, pp. 301-310 (1988).
Saunders and Bush, "Comparison Of Nicotine Biosynthetic Enzymes In Nicotine Level Genotypes Of Burley Tobacco", Agronomy Abstracts, p. 84 (1978).
Saunders and Bush, "Enzyme Activities In Nicotine Biosynthesis In Nicotiana tabacum", Journal of Natural Products, 41, p. 646.
Saunders and Bush, "Nicotine Biosynthetic Enzyme Activities In Nicotiana tabacum L. Genotypes With Different Alkaloid Levels", Plant Physiol., 64, pp. 236-240 (1979).
Sheehy et al., "Reduction Of Polygalacturonase Activity In Tomato Fruit By Antisense RNA", Proc. Natl. Acad. Sci. USA, 85, pp. 8805-8809 (1988).
Smith et al., "Antisense RNA Inhibition Of Polygalacturonase Gene Expression In Transgenic tomatoes", Nature, 334, pp.724-726. (1988).
van der Krol et al., "An Anti-Sense Chalcone Synthase Gene In Transgenic Plants Inhibits Flower Pigmentation", Nature, 333, pp. 866-869 (1988).
van der Krol et al., "Modulation Of Eukaryotic Gene Expression By Complementary RNA Or DNA Sequences", Biotechniques, 6, pp. 958-976 (1988).
van der Krol et al., "Antisense Genes In Plants; An Overview", Gene, 72, pp. 45-50 (1988).
Wagner et al., "The Regulation Of Enzyme Activities Of The Nicotine Pathway In Tobacco", Physiol. Plantarum, 68, pp. 667-672 (1986).
Wagner et al., "Regulation In Tobacco Callus Of Enzyme Activiites Of The Nicotine Pathway", Planta, 168, pp. 408-412 (1986).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of purifying putrescine n-methyltransferase from tobacco does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of purifying putrescine n-methyltransferase from tobacco , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of purifying putrescine n-methyltransferase from tobacco will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-73587

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.