Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-03-31
1994-11-29
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 723, 435 74, 435 75, 435 79, 435960, 435962, 436518, 436548, C12Q 168
Patent
active
053690028
ABSTRACT:
An easy method for detecting injured cell nuclear DNA is provided which does not suffer from "pseudo-positive" staining. The method comprises the steps of subjecting a pathological tissue specimen to acid-hydrolysis to selectively hydrolyze any injured DNA thereby to form single-stranded DNA, then treating the specimen with an anti-single-stranded DNA antibody, and subjecting the specimen to morphological inspection to find the presence of the binding of the antibody to the single-stranded DNA. The binding may be discriminated by a labelling marker on the antibody. Alternatively, a labelled secondary antibody which binds to the anti-ssDNA antibody may be used.
REFERENCES:
Van Loon et al., Immunochemical Detection of DNA Damage Induction and Repair at Different Cellular Stages of Spermatogenesis of the Hamoxer after in Vitro or in Vivo Exposure to Ionizing Radiation. Exptl. Cell Res. 193: 303-309, 1991.
Mezzanotte et al, Cytogenetics and Cell Genetics, 50(1):54-58 (1989).
Dinjens et al, Histochemistry, 98(3):199-205 (1992).
English Language Abstract for JP-A-62-8053.
Kepplinger Esther M.
Maruzen Petrochemical Co. Ltd.
Wortman Donna C.
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