Phenanthridium dye staining of nucleic acids in living cells

Details Phenanthridium dye staining of nucleic acids in living cells Phenanthridium dye staining of nucleic acids in living cells

1993-05-17

1995-08-01

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

054379806

ABSTRACT:
The invention relates to use of fluorescent compounds of the formula: ##STR1## where R contains between 4 and about 10 carbons and is optionally saturated or unsaturated, and is linear or branched or contains an alicyclic or aromatic ring; and the symbol .PSI. depicts the presence of the counterion used to neutralize the positive charge on the dye. The fluorescent dye dissolved in a biologically compatible solution stains a wide variety of living cells with a red nucleic acid stain after brief incubation in low concentrations of dye, without the requirement of permeabilizing reagents. Detection of the fluorescence can be used alone or in combination with measurement of other markers or properties of the cells to identify, discriminate or sort viable cells.

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