Process for storing and recovering plant tissue

Details Process for storing and recovering plant tissue Process for storing and recovering plant tissue

1976-10-12

1977-10-11

Bagwill, Robert E.

Plant husbandry

Process

A01G 100

Patent

active

040528170

ABSTRACT:
Meristematic tissue such as from shoot apices of herbaceous plants in a freezing solution containing a cryoprotectant are cooled to a temperature of at least -70.degree. C, stored when frozen and subsequently thawed at an average warming rate of at least about 24.degree. C/min when the tissue is at a temperature between about -70.degree. C and about -10.degree. C to obtain pathogen-free meristematic tissue having morphogenic potential. The donor plants from which the tissue is to be excised are cold treated to induce cellular reactions believed to be related to the winter cold hardening process, generally at a temperature between the plant freezing temperature and about 10.degree. C.

REFERENCES:
cultivation of Animal & Plant Cells, White, 2nd Ed., 1963, Ronald Press Co., N.Y., p. 180 cited.
3RD Ann. Cong. of Plant Tissue & Cell Culture, Leicester, Eng., 1974, Paper No. 95 relied on, papers 94, 96.

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