Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-12-15
1998-01-20
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 693, 4351723, 435810, 436501, 436 63, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 77, 935 78, 935 88, C12Q 168
Patent
active
057099987
ABSTRACT:
A two-pronged method for diagnosis of genetic diseases can detect mutations in about 87% of familial adenomatous polyposis (FAP) patients. One part of the diagnostic method employs in vitro protein synthesis from surrogate genes created by amplifying either cDNA or genomic DNA. The second part of the diagnostic method employs an allele-specific expression assay which distinguishes the amount of mRNA expressed in vivo from each of a patient's two alleles. These approaches are readily applicable to the diagnosis of other genetic diseases.
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Kinzler Kenneth W.
Vogelstein Bert
Jones W. Gary
Marschel Ardin H.
The Johns Hopkins University
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