Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1995-06-06
1998-01-20
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 6, 435 71, 435 792, 435810, 436 3, 436501, 436172, 436829, 935 77, 935 78, 252700, 252582, G01N 3352, C12Q 168, C12Q 100, C09K 1100
Patent
active
057099944
ABSTRACT:
Methods for labeling a material are disclosed. The methods comprise combining with the material (a) a photosensitizer capable upon irradiation of generating singlet oxygen and (b) a chemiluminescent compound capable of being activated by singlet oxygen wherein the photosensitizer and the chemiluminescent compound are incorporated in a particulate matrix or a non-particulate solid matrix. The particulate matrix can be solid or fluid. The methods allow for generating delayed luminescence, which can be realized upon irradiation of the matrix. The methods have application to the determination of an analyte in a medium suspected of containing the analyte. One method comprises subjecting a medium suspected of containing an analyte to conditions under which a complex of specific binding pair (sbp) members is formed in relation to the presence of the analyte and determining whether the sbp member complex has formed by employing as a label a single composition having both chemiluminescent and photosensitizer properties. Upon activation of the photosensitizer property singlet oxygen is generated and activates the chemiluminescent property. Compositions and kits are also disclosed.
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Kirakossian Hrair
Pease John S.
Ullman Edwin F.
Wagner Daniel B.
Myers Carla J.
Syntex (U.S.A.) Inc.
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