Process for producing subculturable lymphokine-producing human T

Details Process for producing subculturable lymphokine-producing human T Process for producing subculturable lymphokine-producing human T

1984-01-26

1987-06-23

Warren, Charles F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 68, 435240, 935 99, 935101, C12N 1500, C12N 500, C12P 2100

Patent

active

046752951

ABSTRACT:
A process for preparing human T cell hybridomas which are subculturable and produce lymphokines comprising the steps of: (1) treating a human acute leukemia cell with a protein and/or RNA synthesis inhibitor; (2) independently activating a human T cell with a mitogen or antigen; (3) fusing the thus-treated human acute leukemia cell with the thus-activated human T cell in the presence of a fusion accelerator; and (4) isolating the thus-formed hybridoma. An in vivo process for producing lymphokines using the hybridomas is also described.

REFERENCES:
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patent: 4383036 (1983-05-01), Sugimoto et al.
Adams et al., "Leukemia: Serial Transplantation of Human Leukemic Lymphoblasts in the Newborn Syrian Hampster", Cancer Research, 27, pp. 772-783 (1967).
Watanabe et al, "Transplantability of Human Lymphoid Cell Line, Lymphoma and Leukemia in Splenectomized and/or Irradiated Nude Mice", Cancer Research, 40, pp. 2588-2595 (1980).
Grillot-Courvalin et al., "Establishment of Human T-Cell Hybrid Line with Suppressive Activity, Nature, vol. 292, pp. 244-845, 1981.
Grollman, A., "Inhibitors of Protein Biosynthesis", J. of Biol. Chem., vol. 243, pp. 4089-4094, 1968.
Merck Index, Ninth Ed., 1976.
Gillis et al., "Biochemical and Biological Characterization of Lymphocyte Regulatory Molecules", J. Exp. Med., vol. 152, pp. 1709-1719 (1980).
Wright, "Isolation of Heterokaryons and Hybrids by a Selective System", Ex. Cell. Res., vol. 112, pp. 395-407 (1978).
Rocklin, R., "Mediators of Cell Immunity", in Basic and Clinical Immunology (Fudenberg, Ed.) 1978, p. 128.
Hochster et al, "Metabolic Inhibitors", Academic Press, 1963, pp. 30-45.

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