Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1994-07-08
1996-07-23
Robinson, Douglas W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 243, 435 6, C07H 19067, C07H 19167, C07H 2102
Patent
active
055390978
ABSTRACT:
A versatile polymeric support system for the synthesis of oligonucleotides is provided featuring a universal primer which allows chain elongation, in either the 3' or 5' direction, with any currently available DNA or RNA synthesis method, by a process which utilizes oxidatively cleaved primers to facilitate chain elongation and release. The support system is capable of withstanding mildly basic and acidic reaction conditions, while still permitting a convenient and quantitative release, either before or after removal of protecting groups from reactive groups, of synthesized oligonucleotides from a single polymeric support. Removal of the protecting groups before cleavage of the oligomer from the support permits the use of the immobilized oligomer as an affinity hybridization support for both isolating and detecting complementary polynucleic acids.
REFERENCES:
patent: 3440190 (1969-04-01), Melby
patent: 3488405 (1970-01-01), Trementozzi et al.
patent: 3520839 (1970-07-01), Milligan et al.
patent: 3832423 (1974-08-01), Milkovich et al.
patent: 3850749 (1974-11-01), Kaufmann et al.
patent: 3887653 (1975-06-01), Konishi et al.
patent: 3898301 (1975-08-01), Konishi et al.
patent: 4085168 (1978-04-01), Milkovich et al.
patent: 4373071 (1983-02-01), Itakura
patent: 4401796 (1983-09-01), Itakura
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 4500707 (1985-02-01), Caruthers et al.
Matteucci et al, "Synthesis of Deoxynucleotides on a Polymer Support," J. Am. Chem. Soc., 103(11), 3185-3191 (1981).
Sommer et al., "Chemische Syntheses von Desoxyoligonucleotiden mit 5'-Phosphatgruppe am Polymeren Tr ager," Chem. Ber., 107, 24-33 (1974).
Koster (I), "Polymer Support Oligonucleotide Synthesis VI. Use of Inorganic Carriers," Tetrahedron Letters, 1972, (16), 1527-1530.
Koster (II), "Polymer Support Oligonucleotide Synthesis VII. Use of Sephadex LH 20," Tetrahedron Letters, 1972, (16), 1531-1534.
Koster (III), "Die Bedeutung der Chemischen Synthesis von DNA f ur die Gentechnologie," Arzneim.-Forsch/Drug Res., 30, 548-557 (1980).
Matteucci et al. (II), "The Synthesis of Oligodeoxypyridines on a Polymer Support," Tetrahedron Letters, 21, 719-722 (1980).
Arshady et al., "Easily Prepared Polar Support for Solid Phase Peptide and Oligonucleotide Synthesis. Preparation of Substance P and a Nonadeoxyribonucleotide," J. Chem. Soc. Chem. Comm., 1979, 423-425.
Dunn et al., "Mapping Viral mRNAs by Sandwich Hybridization," Methods in Enzymology, 65, 468-478 (1980).
Ranki et al., "Sandwich Hybridization as a Convenient Method for the Detection of Nucleic Acids in Crude Samples," Gene, 21, 77-85 (1983).
Palva et al., "ompA Gene in the Detection of Escherichia coli and Other Enterobacteriaceae by Nucleic Acid Sandwich Hybridization," J. Clin. Microbiol., 18, 92-100 (1983).
Bunemann, "Immobilization of Detatured DNA to Microporous Supports: II. Steric and Kinetic Parameters of Heterogeneous Hybridization Reactions," Nucleic Acids Research, 10, 7181-7196 (1982).
Hayakawa et al., "The Allylic Potection Method in Solid-Phase Oligonucleotide Synthesis. An Efficient Preparation of Solid-Anchored DNA Oligomers," J. Am Chem. Soc., 112, 1691-1696 (1990).
Chow et al., "A High Capacity, Reusable Oligodeoxythymidine Affinity Column," Analytical Biochemistry, 175, 63-66 (1988).
Blanks et al., "An Oligonucleotide Affinity Column for the Isolation of Sequence Specific DNA Binding Proteins," Nucleic Acids Research, 16(21), 10283-10298 (1988).
Duncan et al., "Affinity Chromatography of a Sequence-Specific DNA Binding Protein Using Teflon-Linked Oligonculeotides," Analytical Biochemistry, 169, 104-108 (1988).
Arnold et al., "A Novel Universal Support for DNA & RNA Synthesis," Federation Proceedings, 43(7), Abstr. No. 3669 (1984).
Lohrmann et al., "New Solid Supports for DNA Synthesis," DNA, 3(1), 122 (1984).
Kochetkov et al.(eds.), "Organic Chemistry of Nucleic Acids, Part A," Plenum Press, New York, New York, 1971, see pp. 48-51, see particularly p. 49.
March, "Advanced Organic Chemistry: Reactions, Mechanism and Structure," McGraw-Hill Book Company, New York, NY, 1968, see p. 875.
Ikehara et al. eds., "The Synthesis of Polynucleotides," Adv. Carbohydrate Chem. and Biochem., 36, 135-213, only 202, 207-213 supplied (1979).
Kochetkov et al., "Structure of Nucleic Acids," Chapter 1 in Organc Chemistry of Nucleic Acids, Part A, Plenum Press, New York, NY, 1971, only pp. 48-49 and 91 supplied.
Crane L. Eric
Molecular Biosystems, Inc.
Robinson Douglas W.
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