Method for preparing fertile transgenic corn plants

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4351721, 43524048, 43524049, 935 52, 935 55, 935 67, 935 85, 800205, 800235, 800DIG56, C12N 1500, C12N 1505, A01H 106, A01H 400

Patent

active

055388809

ABSTRACT:
Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to be transformed. The process may be applicable to other graminaceous cereal plants which have not proven stably transformable by other techniques.

REFERENCES:
patent: 4370160 (1983-01-01), Ziemelis
patent: 4559302 (1985-12-01), Ingolia
patent: 4581847 (1986-04-01), Hibberd et al.
patent: 4665030 (1987-05-01), Close
patent: 4666844 (1987-05-01), Cheng
patent: 4727028 (1988-02-01), Santerre et al.
patent: 4806483 (1989-02-01), Wang
patent: 4940835 (1990-07-01), Shah et al.
patent: 5049500 (1991-09-01), Arnizen et al.
"Bullets" Transform Plant Cells, Agricell Report, 9, 5 (Jul. 1987).
"Shotgunning DNA into Cells," Genetic Engineering News, (Jul./Aug. 1987).
Ahokes, H. "Electrophoretic transfection of cereal grains with exogenous nucleic acid," Soc. Biochem. Biophys. Microbio. Fen., Biotieteen Paivat (Bioscience Days), Technical University of Helsinki, Espoo, p. 2 (1989).
Armstrong, C. L., et al., "Genetic and cytogenetic variation in plants regenerated from organogenic and friable, embryonic tissue cultures of maize," Biological Abstracts, vol. 85, Abstract No. 117662 (1988).
Barker, R. F., et al., "Nucleotide Sequence of the T-DNA Region from the Agrobacterium tumefaciens Octopone Ti Plasmid pTil5955," Plant Mol. Biol., 2, 335-350 (1983).
Bevan, M., et al., "A Chimaeric Antibiotic Resistance Gene as a Selectable Marker for Plant Cell Transformation," Nature, 304, 184-187 (1983).
Bevan, M., et al., "Structure and Transcription of the Nopaline Synthase Gene Region of T-DNA," Nuc. Acids Res., 11, 369-385 (1983).
Booy, G., et al., "Attempted Pollen-Mediated Transformation of Maize," J. Plant Physiol., 135, 319-324 (1989).
Callis, J., et al., "Introns Increase Gene Expression in Cultures Maize Cells," Genes and Development, 1, 1183-1200 (1987).
Cao, J., et al., "Transformation of Rice and Maize using the Biolistic Process," In: Plant Gene Transfer, Alan R. Liss, Inc., pp. 21-33 (1990).
Chandler, V. L., et al., "Two Regulatory Genes of the Maize Anthocyanin Pathway are Homologous Isolation of B Utilizing R Genomic Sequences," The Plant Cell, 1, 1175-1183 (1989).
Christou, P., et al., "Cotransformation Frequencies of Foreign Genes in Soybean Cell Cultures," Theor. Appl. Genet., 79, 337-341 (1990).
Christou, P., et al., "Stable Transformation of Soybean Callus by DNA-Coated Gold Particles," Plant Physiol., 87, 671-674 (1988).
Cocking, F., et al., "Gene Transfer in Cereals," Science, 236, 1259-1262 (1987).
Creissen, G., et al., "Agrobacterium-and Microprojectile-Mediated Viral DNA Delivery into Barley Microspore Derived-Cultures," Plant Cell Rep., 8, 680-683 (Apr. 1990).
Crossway, A., et al., "Integrated of foreign DNA following microinjection of tobacco mesophyll protoplasts," Mol. Gen. Genet., 202, 179-185 (1986).
De Block, M., et al., "Engineering herbicide resistance on plants by expression of a detoxifying enzyme," EMBO J., 6, 2513-2518 (1987).
De Greef, W., et al., "Evaluation of herbicide resistance in transgenic crops under field conditions," Bio/Technol., 7, 61-64 (1989).
Dekeyser, R. A., et al., "Evaluation of Selectable Markers for Rice Transformation," Plant Physiol., 90, 217-223 (1989).
DeWald et al., "Plant regeneration from inbred maize suspensions," VIIth International Congress on Plant Tissue and Cell Culture, p. 12, Abstract No. A1-36 (Jun. 24-29, 1990).
DeWet, J. R. et al., "Cloning of Firefly Luciferase cDNA and the Expression of Active Lucifease in Escherichia coli," Proc. Nat. Acad. Sci, USA, 82, 7870-7873 (1985).
Evans, D. A., et al., "Somaclonal Variation--Genetic Basis and Breeding Applications," Trends Genet., 5, 46-50 (1989).
Fransz, P., et al., "Cytodifferentiation during callus initiation and somatic embryogenesis in Zea mays L.," Ph.D. thesis, U. of Wageningen Press, The Netherlands (1988).
Fromm, M. E., et al., "Stable Transformation of Maize after Gene Transfer by Electroporation," Nature, 319, 791-793 (1986).
Fromm, M., et al., "Expression of Genes Transfected into Monocot and Dicot Plant Cells by Electroporation," Proc. Nat. Acad. Sci. USA, 82, 5824-5828 (1985).
Gould, O., et al., "Shoot Tip Culture as a Potential Transformation System," Abstracts, Beltwide cotton production research conferences, New Orleans, LA, p. 91 (1988).
Graves, A., et al., "The transformation of Zea mays seedlings with Agrobacterium tumefacians", Plant Mol. Biol., 7, 43-50 (1986).
Green, C., et al., "Plant Regeneration from Tissue Cultures of Maize," Crop. Sci., 15, 417-421 (1975).
Green, C., et al., "Plant Rengeneration in Tissue Cultures of Maize," In: Maize for Biological Research, Sheridan, W. F., (ed.) Plant Mol. Biol. Assoc., pp. 367-372 (1982).
Green, C., et al., "Somatic Cell Genetic Systems in Corn," In: Advances in Gene Technology: Molecular Genetics Plant and Animals, Academic Press, Inc., pp. 147-157 (1983).
Grimsley, N., et al., "DNA Transfer from Agrobacterium to Zea mays or Brassica by Agroinfection is Dependent on Bacterial Virulence Functions," Mol. Gen. Genet., 217, 309-316 (1989).
Gritz, L., et al., "Plasmid-Encoded Hygromycin B Resistance: The Sequences of Hygromycin B Phosphotransferase Gene and Its Expression in Escherichia coli and Saccharomyces cerevisiae," Gene, 25, 179-188 (1983).
Guilley, H., et al., "Transcription of Cauliflower Mosaic Virus DNA: Detection of Promotor Sequences, and Characterization of Transcripts," Cell, 30, 763-773 (Oct. 1982).
Hooykaas, P. J. J., "Transformation of plant cell via Agrobacterium," Plant Mol. Biol., 13, 327-336 (1989).
Horn, M., et al., "Transgenic Plants of Orchard Grass (Dactylis glomerata L.) from Protoplasts," Chem. Abstracts, 110, p. 208, Abstract No. 89869a (1989).
Jefferson, R., et al., ".beta.-Glucuronidase from Escherichia coli as a Gene-Fusion Marker," Proc. Nat. Acad. Sci. USA 83, 8447-8451 (1986).
Jefferson, R., et al., "GUS Fusions: .beta.-Glucuronidase as a Sensitive and Versatile Gene Fusion Marker in Higher Plants," EMBO J. 6, 3901-3907 (1987).
Jefferson, R., "Assaying chimeric genes in plants: the GUS gene fusion system," Plant Mol. Biol. Rep., 5, 387-405 (1987).
Kamo, K., et al., "Establishman and Characterization of Long-Term Embryonic Maize Callus and Cell Suspension Cultures," Plant Sci., 45, 111-117 (1986)
Kartha, K., et al., "Transient Expression of Chloramphenicol Acetyl Transferase (CAT) Gene in Barley Cell Cultures and Immature Embryos Through Microprojectile Bombardment," Plant Cell Rep., 8, 429-432 (1989).
Klein, T., et al., "Transfer of Foreign Genes into Intact Maize Cells with High-Velocity Microprojectiles," Proc. Nat. Acad. Sci. USA, 85, 4305-4309 (1988).
Klein, T. M., et al., "Factors Influencing Gene Delivery into Zea mays Cells by High Velocity Microprojectiles," Bio/Technol., 6, 559-563 (1988).
Klein, T. M., et al., "High-Velocity Microprojectiles for Delivering Nucleic Acids to Living Cells," Nature, 327, 70-73 (1987).
Klein, T., et al., "Genetic Transformation of Maize Cell by Particle Bombardment and the Influence of Methylation on Foreign Gene Expression," in: Gene Manipulation in Plant Improvement II, Gustafson, J. P., (ed.), Plenum Press, NY, pp. 265-266 (1990).
Klein, T., et al., "Genetic transformation of Maize Cells by Particle Bombardment," Plant Physiol., 91, 440-444 (1989).
Klein, T., et al., "Regulation of Anthocyanin Biosynthetic Genes Introduced into Intact Maize Tissue by Microprojectiles," Proc. Nat. Acad. Sci. USA, 86, 6682-6685 (1989).
Kozak, M., "Point Mutations Define a Sequence Flanking the AUG Initiator Codon that Modulates Translation by Eukaryotic Ribosomes," Cell, 44, 283-292 (1986).
Lazzeri, P., et al., "In Vitro Genetic Manipulation of Cereals and Grasses," Ad. Cell Culture 6, 291-293 (1988).
Lorz, H., et al., "Advances in Tissue Culture and Progress Towards Genetic Transformation of Cereals," Plant Breeding, 100, 1-25 (1988).
Lu, C., et al., "Improved Efficiency of Somatic Embryogenesis and Plant Reg

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for preparing fertile transgenic corn plants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for preparing fertile transgenic corn plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for preparing fertile transgenic corn plants will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-712331

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.