Procedure for the simultaneous quantification, in a single measu

Details Procedure for the simultaneous quantification, in a single measu Procedure for the simultaneous quantification, in a single measu

1993-12-09

1996-07-23

Housel, James C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 725, 436536, 436546, 436172, 436800, G01N 33533, G01N 33536, G01N 33577

Patent

active

055388558

ABSTRACT:
Procedure for the simultaneous quantification, in a single measurement, of the major types of human lymphocytes and their subsets. It includes: incubation of the sample with five different monoclonal antibodies conjugated with three different fluorochromes; measurement by flow cytometry of the three fluorescent emissions; and analysis of the results obtained by means of multiparametric analysis to determine the presence/absence of a certain fluorescence in a cell and its intensity in each cell.

REFERENCES:
patent: 4499052 (1985-02-01), Fulwyler
Rabian-Betzog, C. et al, "Characterization of lymphocyte subpopulations in cord blood", Bone marrow Transplant, vol. 9(Supplement 1), pp. 64-67, Jun. 1992
Mandy, F. F., et al, "A simultaneous three-color T cell subsets analysis with single laser flow cytometers using T cell gating protocol", Journal of Immunological Methods, vol. 156, pp. 151-162, Dec. 8, 1992.
Richards, S. J. et al, "Immunophenotypic Dissection of Normal Peripheral Blood NK Associated (NKa) Subpopulations by Flow Cytometry: Morphological Features and Relationships Between Membrane NKa (CD11b, CD16, CD56 and CD57) and T-Cell (CD2, CD3, TCR, CD5, CD7, CD8 and CD38) Associated Determinant Expression", Leukemia and Lymphoma, vol. 2, pp. 111-126, 1990.
Lymphocyte Subset Reference Ranges in Adult Caucasians, T. Reichert, et al., Clinical Immunology and Immunopathology 60, 190-208, (1991).

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