Vectors suitable for detection of eukaryotic DNA regulatory sequ

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 68, 4351723, 435320, 4352402, 935 9, 935 27, 935 32, 935 70, 935 71, 935 77, C12Q 168, C12N 1500, C12N 500, C12P 2100

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047613670

ABSTRACT:
The present invention relates to vectors useful for identifying eukaryotic regulatory sequences and to a method for identifying these sequences. The vectors comprise a first nucleotide sequence which allows for replication in a eukaryotic host, a second nucleotide sequence which codes for a first product that controls replication at said first sequence, and a third nucleotide sequence which codes for a second product that is detectable. The method comprises inserting putative eukaryotic regulatory sequences at a position on the 5'-side of the second sequence and measuring the production of the second product.

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