Method of isolating purified plasmid DNA using a nonionic deterg

Details Method of isolating purified plasmid DNA using a nonionic deterg Method of isolating purified plasmid DNA using a nonionic deterg

1994-08-26

1997-04-29

Wilson, James O.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 254, 210634, 210638, 210511, C07H 106

Patent

active

056250533

ABSTRACT:
A method of isolating purified plasmid DNA includes the steps of providing cells containing plasmid DNA and lysing the cells with an alkyldimethylphosphine oxide (APO) detergent to produce a mixture, centrifuging the cell and APO mixture, reserving a supernatant containing purified plasmid DNA and isolating the purified plasmid DNA, from the supernatant. Isolation and purification occurs in the absence of hazardous materials such as phenol and chloroform. Prior to mixing, the APO detergent is preferably combined with a strong base to produce a stable solution.

REFERENCES:
patent: 5271840 (1993-12-01), Kresheck
Maniatis, Molecular Cloning, A Laboratory Manual, pp. 90-91, (1982).

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