Mutations in the gene encoding the alpha chain of platelet glyco

Details Mutations in the gene encoding the alpha chain of platelet glyco Mutations in the gene encoding the alpha chain of platelet glyco

1994-04-28

1997-04-29

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 696, 435 698, 435 701, 435 703, 4351721, 4351723, 435348, 4352523, 43525233, 4353201, 435 6, 435361, 536 231, 536 235, 536 2431, 935 10, 935 27, 935 56, 935 57, 935 70, 935 73, 935 78, C12N 1512, C12N 1511, C12N 1563, C12N 510

Patent

active

056248172

ABSTRACT:
The subject invention provides purified polypeptides encoded by naturally-occurring wild-type platelet glycoprotein Ib alpha having a mutation which renders the polypeptide more reactive with yon Willebrand factor. Preferably, the mutation is in the hinge region of GP Ib.alpha., such as the substitution of valine for glycine at residue 233. These mutations alter the three-dimensional structure of the mutant polypeptide from a beta bend conformation to an alpha helix formation, and also create an amphipathic region within the mutant polypeptide. DNA encoding the mutant polypeptides, as well as expression systems for the production of the mutant polypeptides, are also provided. Methods and compositions using the mutant polypeptides and DNA oligomers complementary to the mutant polypeptides are further provided.

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