Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-03-14
1999-09-28
Feisee, Lila
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 71, 435 77, 435 691, 435183, 4353201, 435325, 4352523, 43525411, 536 231, 536 235, 536 234, C12Q 100, C12N 510, C12N 1512, C07K 14435
Patent
active
059587130
ABSTRACT:
The present invention provides a method for detecting a biologically active substance that affects intracellular processes mediated through a protein kinase or second messenger, which comprises incubating a cell with a test substance and measuring any change caused by the test substance in the fluorescence of (i) green fluorescent protein (GFP) having a protein kinase recognition site or (ii) GFP that has been modified to contain a second messenger binding domain.
REFERENCES:
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patent: 5683888 (1997-11-01), Campbell
Martin Chalfie et al., "Green Fluorescent Protein As A Marker For Gene Expression", Science vol. 263, Feb. 11, 1994, pp. 802-805.
Sala-Newby et al. Engineering a bioluminescent indicator for cyclic AMP-dependent protein kinase. Biochemical Journal. vol. 279, pp. 727-732, 1991.
Sala-Newby et al. Engineering firefly luciferase as an indicator of cyclic AMP-dependent protein kinase in living cells. FEBS Letters. vol. 307, No. 2, pp. 241-244, Jul. 1992.
Sala-Newby et al. Engineering protein kinase recognition sites into firefly luciferase and aequorin. In Biolumin. Chemilumin. Proc. Int. Symp., Stanely et al., eds. Wiley, UK, pp. 23-26, 1991.
Campbell et al. Engineering bioluminescent proteins: a new experimental strategy for measuring intracellular signalling in live cells. Methodol. Surv. Biochem. Anal. vol. 21, pp. 311-316, 1991.
Bj.o slashed.rn Sara Petersen
Poulsen Lars Kongsbak
Thastrup Ole
Tullin S.o slashed.ren
Feisee Lila
Kaufman Claire M.
Novo Nordisk A S
Villacorta Gilberto M.
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