Protein activity screening of clones having DNA from uncultivate

Details Protein activity screening of clones having DNA from uncultivate Protein activity screening of clones having DNA from uncultivate

1996-06-03

1999-09-28

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435183, 435 691, 536 231, 536 232, C12Q 100, C12N 900

Patent

active

059586729

ABSTRACT:
Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein, e.g. enzyme, activity.

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Zhou, Y. et al. (1991) "Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase" Nucleic Acids Research, vol. 19, No. 21, p. 6052, Nov. 1991.
Kirshtein, J.D. et al. (1991) "Amplification, cloning, and sequencing of a nifH segment from aquatic microorganisms and natural communities" Applied and Environmental Microbiology, vol. 57, No. 9, pp. 2645-2650, Sep. 1991.
Ueda, T. et al. (1995) "Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolution analysis of nifH gene sequences" Journal of Bacteriology, vol. 177, No. 5, pp. 1414-1417, Mar. 1995.
Hennecke, H. et al. (1985) "Concurrent evolution of nitrogenase genes and 16S rRNA in Rhizobium species and other nitrogen fixing bacteria" Archives of Microbiology, vol. 142, pp. 342-348, 1985.

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