Methods of producing hybrid G protein-coupled receptors

Details Methods of producing hybrid G protein-coupled receptors Methods of producing hybrid G protein-coupled receptors

1990-02-08

1994-02-08

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4352401, 435 29, 4351723, 435 681, C12N 1500, C12N 500, C12Q 168, C12P 2100

Patent

active

052847462

ABSTRACT:
Methods are disclosed for producing hybrid G protein-coupled receptors. DNA sequences encoding hybrid G protein-coupled receptors are provided, wherein the receptors comprise mammalian G protein-coupled receptors having at least one domain other than the ligand-binding domain replaced with a corresponding domain of a yeast G protein-coupled receptor. DNA constructs comprising the following operatively linked elements: a transcriptional promoter, a DNA sequence encoding a hybrid G protein-coupled receptor, wherein the receptor comprises a mammalian G protein-coupled receptor having at least one domain other than the ligand-binding domain replaced with a corresponding domain of a yeast G protein-coupled receptor, and a transcriptional terminator. Host cells transformed with the DNA constructs and methods utilizing the transformed cells are also provided.

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