Sample well insert with wedge-shaped profile for ultra-thin slab

Details Sample well insert with wedge-shaped profile for ultra-thin slab Sample well insert with wedge-shaped profile for ultra-thin slab

1992-12-23

1994-02-08

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, G01N 2726, G01N 27447

Patent

active

052845656

ABSTRACT:
A comb to be used for holding samples and defining sample lanes in an ultra-thin slab gel in vertical slab gel electrophoresis is constructed with to form tapered or wedged-shaped sample wells, with the narrowest edge of the wedge opening into the upper edge of the gel. In one embodiment, the comb has a wedge-shaped profile along the contact edge to provide an expanded well at each sample location. Notches alternating with grooves are cut into the wedge shape to form teeth which taper along orthogonal axes, the wells being defined by the space between each adjacent pair of teeth. The gel enclosure which holds the slab gel is formed by plates which are shaped along one edge to provide a complementary wedge-shaped opening leading into the gel space. The comb fits snugly into the opening with the teeth of the comb touching or extending a short distance into the upper exposed surface of the gel. In another embodiment, the comb has a rectangular profile, with grooves cut to a varying depth to form the wedge shape.

REFERENCES:
patent: 4883577 (1989-11-01), Sugimoto et al.
patent: 5073246 (1991-12-01), Chu et al.
patent: 5164065 (1992-11-01), Bettencourt et al.
Douglas E. Kolner "Ultrathin DNA Sequencing Gels Using Microtrough Vertical Electrophoresis Plates" BioTechniques vol. 13, No. 3 (Sep. 1992) 338-339.

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