Electrophoresis of nucleic acid fragments

Details Electrophoresis of nucleic acid fragments Electrophoresis of nucleic acid fragments

1992-08-10

1998-11-03

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 435 915, 435183, 436 94, 935 77, 204450, 204456, C12Q 168, C12P 1934, G01N 2726

Patent

active

058306420

ABSTRACT:
A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2% or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.

REFERENCES:
patent: 4795699 (1989-01-01), Tabor
patent: 4844786 (1989-07-01), Sugihara
patent: 5041371 (1991-08-01), Cowan et al.
patent: 5075216 (1991-12-01), Innis
patent: 5314809 (1994-05-01), Erlice
Thivierge, Bethany, Amplification: A Forum for PCR Users, 1989, Issue 2, pp. 9-10.
Perkei elmer Catalog (1992) pp. 5, 9, 11, 64 & 65.
Lantick et al DNA (1984) 3:413-419.
Wilson et al Genomics (1990) 6:626-634.
Stoll et al, Methods in Enzynology, Guide to Protein Purification, 1990 Academic Press, Inc, vol. 182, pp. 24-31.
Thangavelu et al Cytogenet Cell Genet 1991 58(1-4) 2154.
Hong, Bioscience (1982) 2:907-912.
Straus et al BioTechniques (Mar. 1991)10: 376-384.
U.S. Biochemical Catalog (1990) pp. 94, 115&116.
Schleif et al Practical Methods in Moleculer Biology, 1981 Springer-Verlag, new York, NY, pp. 114-119.
Pharmacia Catlog, pp. 120-123, 184.
Mamatis et al. Molecular Cloning: A Laboratory Manual, 1st Ed, 1982, Cold Spring Harbor Laboratory Press, NY, pp. 104-106.
Carninci et al., A simple discontinuous buffer system for increased resolution and speed in gel electrophoretic analysis of DNA sequencing, 18 Nucleic Acids Research 204, 1989.
Richards et al., Disc Electrophoresis of Ribonucleic Acid in Polyacrylamide Gels, 12 Anal. Biochem. 452, 1965.
Peacock et al., "Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis", 6 Biochemistry 1818, 1967.
Ansorpe et al., "System for DNA sequencing with resolution of up to 600 base pairs", 9 J. Bioc. Biop. Meth. 33, 1984.
Brumley et al., "Rapid DNA sequencing by horizontal ultrathin gel electrophoresis", 19 Nucleic Acids Research 4121, 1991.
Wyckoff et al., "Polyacrylamide Gel Electrophoresis in sodium Dodecyl Sulfate-Containing Buffers Using Multiphasic Buffer Systems: Properties of the Stack, Valid R.sub.f -Measurement, and Optimized Procedure", 78 Anal. Biochem. 459, 1977.
Fuller, "The Effect of Excess Glycerol on DNA Sequencing Gels", USB Comments (brochure).

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