Electrophoresis of nucleic acid fragments

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 91, 435 915, 435183, 436 94, 935 77, 204450, 204456, C12Q 168, C12P 1934, G01N 2726

Patent

active

058306420

ABSTRACT:
A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2% or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or other sugars, which interact to form a complex with borate or boric acid. The method includes applying the solution to an electrophoretic gel and electrophoresing those fragments into the gel in the presence of a buffer lacking boric acid, or a derivative thereof, which forms a chelate complex with the reagent and thereby causes distortion of electrophoresis of the fragments in a gel including such a buffer.

REFERENCES:
patent: 4795699 (1989-01-01), Tabor
patent: 4844786 (1989-07-01), Sugihara
patent: 5041371 (1991-08-01), Cowan et al.
patent: 5075216 (1991-12-01), Innis
patent: 5314809 (1994-05-01), Erlice
Thivierge, Bethany, Amplification: A Forum for PCR Users, 1989, Issue 2, pp. 9-10.
Perkei elmer Catalog (1992) pp. 5, 9, 11, 64 & 65.
Lantick et al DNA (1984) 3:413-419.
Wilson et al Genomics (1990) 6:626-634.
Stoll et al, Methods in Enzynology, Guide to Protein Purification, 1990 Academic Press, Inc, vol. 182, pp. 24-31.
Thangavelu et al Cytogenet Cell Genet 1991 58(1-4) 2154.
Hong, Bioscience (1982) 2:907-912.
Straus et al BioTechniques (Mar. 1991)10: 376-384.
U.S. Biochemical Catalog (1990) pp. 94, 115&116.
Schleif et al Practical Methods in Moleculer Biology, 1981 Springer-Verlag, new York, NY, pp. 114-119.
Pharmacia Catlog, pp. 120-123, 184.
Mamatis et al. Molecular Cloning: A Laboratory Manual, 1st Ed, 1982, Cold Spring Harbor Laboratory Press, NY, pp. 104-106.
Carninci et al., A simple discontinuous buffer system for increased resolution and speed in gel electrophoretic analysis of DNA sequencing, 18 Nucleic Acids Research 204, 1989.
Richards et al., Disc Electrophoresis of Ribonucleic Acid in Polyacrylamide Gels, 12 Anal. Biochem. 452, 1965.
Peacock et al., "Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis", 6 Biochemistry 1818, 1967.
Ansorpe et al., "System for DNA sequencing with resolution of up to 600 base pairs", 9 J. Bioc. Biop. Meth. 33, 1984.
Brumley et al., "Rapid DNA sequencing by horizontal ultrathin gel electrophoresis", 19 Nucleic Acids Research 4121, 1991.
Wyckoff et al., "Polyacrylamide Gel Electrophoresis in sodium Dodecyl Sulfate-Containing Buffers Using Multiphasic Buffer Systems: Properties of the Stack, Valid R.sub.f -Measurement, and Optimized Procedure", 78 Anal. Biochem. 459, 1977.
Fuller, "The Effect of Excess Glycerol on DNA Sequencing Gels", USB Comments (brochure).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Electrophoresis of nucleic acid fragments does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Electrophoresis of nucleic acid fragments, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Electrophoresis of nucleic acid fragments will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-687819

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.