Synthetic resins or natural rubbers -- part of the class 520 ser – Synthetic resins – Mixing of two or more solid polymers; mixing of solid...
Patent
1995-05-30
1998-09-29
Teskin, Fred
Synthetic resins or natural rubbers -- part of the class 520 ser
Synthetic resins
Mixing of two or more solid polymers; mixing of solid...
525 54, 536 253, 536 2531, 536 2534, 530334, 530335, C07K 104
Patent
active
058147004
ABSTRACT:
A method of synthesis for building a polymer chain, oligonucleotides in particular, by sequentially adding monomer units to at least one solid support for growing and immobilizing a polymer chain thereon in a liquid reagent solution. The method includes the step of: A) depositing a liquid reagent in a reaction well (26) in contact with at least one solid support and at least one monomer unit of the polymer chain affixed to the solid support. The well (26) includes at least one orifice (74) extending into the well (26), and is of a size and dimension to form a capillary liquid seal to retain the reagent solution in the well (26) to enable polymer chain growth on the solid support. The method further includes the step of B) expelling the reagent solution from the well (26), while retaining the polymer chain therein. This is accomplished by applying a first gas pressure to the reaction well such that a pressure differential between the first gas pressure and a second gas pressure exerted on an exit (80) of the orifice (74) exceeds a predetermined amount sufficient to overcome the capillary liquid seal and expel the reagent solution from the well (26) through the orifice exit (80).
REFERENCES:
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 4500707 (1985-02-01), Caruthers et al.
patent: 4517338 (1985-05-01), Urdea et al.
patent: 5047524 (1991-09-01), Andrus et al.
patent: 5106583 (1992-04-01), Raysberg et al.
patent: 5378776 (1995-01-01), Matsumura et al.
patent: 5538694 (1996-07-01), Delius
Bray, et al., "Direct Cleavage of Peptides from a Solid Support into Aqueous Buffer. Application in Simultaneous Multiple Peptide Synthesis", J. Org. Chem., 56:6659-6666 (1991).
Fodor, et al., "Light-Directed, Spatially Addressable Parallel Chemical Synthesis", Science, 251:767-773 (1991).
Khrapko, et al., "An oligonucleotide hybridization approach to DNA
"Oligonucleotides and Analogues:A Practical Approach", F. Eckstein, Ed., IRL Press at Oxford University Press.
Brezner David J.
Teskin Fred
The Board of Trustees of the Leland Stanford Junior University
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