Colloidal silica films for cell culture

Details Colloidal silica films for cell culture Colloidal silica films for cell culture

1996-09-26

1998-09-29

Guzo, David

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Solid support and method of culturing cells on said solid...

4352891, 4353051, C12M 304

Patent

active

058145508

ABSTRACT:
Methods for producing thin colloidal silica films on substrates, such as corona treated polystyrene, are provided. The dried films are characterized as 50 nm thick, high silanol, homogenous, high surface area, porous, uncracked, adherent, wetting, negatively charged, and gamma radiation stable. Several differential advantages of the colloidal silica films were demonstrated in epithelial cell culture, especially regarding primary cultures in serum free media. Cell responses to the films were increased explant adhesion, increased cell growth rate, and increased expression of differentiated function before and after subculture as compared to tissue culture polystyrene.

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patent: 4448884 (1984-05-01), Henderson
patent: 4661407 (1987-04-01), Henderson
Barnes et al. Methods for Growth of Cultured Cells in Serum-Free Medium. Analytical Biochemistry, 1980, vol. 102, pp. 255-270, entire document.

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