Protein involved in regenerating firefly luciferin

Details Protein involved in regenerating firefly luciferin Protein involved in regenerating firefly luciferin

1997-06-05

1998-09-29

Weber, Jon P.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 8, 530417, C12N 962, C12Q 166, A63J 100

Patent

active

058145044

ABSTRACT:
A purified protein having a molecular weight of 40 kD by SDS-PAGE that produces firefly luciferin when combined with D-cysteine and firefly oxyluciferin and isolated from firefly species is provided, as well as methods of making and using the protein for the continuous regeneration of of firefly luciferin.

REFERENCES:
Strehler, "Bioluminescence assay. Principles and practice", Method Biochem. Anal. (1968) 16: 99-181.
"New Riverside University Dictionary"(Riverside Publishing Co., Boston) (1994) p. 480 and 680.
Johnson et al. "Bacterial and other 'luciferins'", Bioscience (1975) 25(11): 718-722, Nov. 1975.
Okada et al. "Firefly Bioluminescence III. Conversion of oxyluciferin to luciferin in firefly", Tetrahedron Lett. (1974) 32: 2771-2774.
McCapra et al. "Biosynthesis of luciferin in Pyrophorus pellucens", J. Chem. Soc. Comm. (1976) 5: 153-154, Mar. 1976.

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