Method and reagent for quantitative determination of N-acetylhex

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase

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435 4, 435 28, 435 18, 435810, C12Q 126, C12Q 100, C12Q 128, C12Q 134

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046346635

ABSTRACT:
A method for quantitative determination of N-acetylhexosamine which comprises (a) allowing an N-acetylhexosamine oxidase to act upon an N-acetylhexosamine-containing liquid in the presence of oxygen and then (b) determining the amount of oxygen consumed in the oxidation reaction or the amount of hydrogen peroxide or N-acetylhexaminic acid formed in the oxidation reaction. Further, a reagent for quantitative determination of N-acetylhexosamine, characterized by containing an N-acetylhexosamine oxidase.

REFERENCES:
Chemical Abstracts, 101: 226338g, 405 (1984).
Chemical Abstracts, 102: 60787w, 481 (1985).

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