Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preparation composition
Patent
1990-07-02
1997-02-04
Le, Long V.
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Preparation composition
436 65, 436501, 436510, 436518, 436531, 436808, 436811, 435 71, 435810, G01N 3353
Patent
active
055997155
ABSTRACT:
An aqueous wash solution is buffered to a pH of from about 5 to about 9 and contains at least about 1.5 weight percent of a compound comprising a lower alcohol sulfate anion having from 6 to 10 carbon atoms and an alkali metal or ammonium cation, such as sodium decyl sulfate. This wash solution is useful in a method for the determination of an immunological ligand, and is not prone to crystallization at lower temperatures. Particularly, it is useful for washing the immunological complex formed between the ligand and a receptor molecule therefor. Unreacted materials can be readily separated from the complex by the washing, particularly if the separation is carried out using a filtration membrane in a test device. A test kit for ligand determination comprises the wash solution as well as one or more receptors for the ligand, at least one of which is labeled for detection. This kit is particularly useful for measuring human chorionic gonadotropin (hCG) as an early indicator of pregnancy.
REFERENCES:
patent: 4663291 (1987-05-01), Rose
patent: 4757134 (1988-07-01), Blake et al.
patent: 4816440 (1989-03-01), Thompson
patent: 4960691 (1990-10-01), Gordon et al.
patent: 4965191 (1990-10-01), Warren, III et al.
patent: 5017474 (1991-05-01), McClune et al.
T. W. Graham Solomons, Organic Chemistry, 1978, pp. 95-97.
Roberts et al., Basic Principles of Organic Chemistry, 2nd edition, 1977, pp. 803-805.
Maniatis et al., Molecular Cloning, A Laboratory Manual 1982, pp. 191 and 445-447.
Odell et al., Clin. Chem., 32 (10), pp. 1873-1878 (1986).
Norkus Norbert S.
Smith-Lewis Margaret J.
Warren, III Harold C.
Johnson & Johnson Clinical Diagnostics Inc.
Le Long V.
Wallen III John W.
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