Chemistry: analytical and immunological testing – Measurement of electrical or magnetic property or thermal... – By means of a solid body in contact with a fluid
Patent
1985-08-23
1987-01-06
Terapane, John F.
Chemistry: analytical and immunological testing
Measurement of electrical or magnetic property or thermal...
By means of a solid body in contact with a fluid
422 68, 422 98, 436178, G01N 2750
Patent
active
H00002011
ABSTRACT:
A compact and portable biosensor which can detect the presense of an environmental stimulus is produced by extracting cell membrane proteins which are specific for the stimulus, purifying the proteins, and reincorporating the proteins into synthetic membranes. Electrodes are used to measure the voltage or current changes across the membrane when the proteins in the synthetic membranes react to the specific stimulus. The magnitude of the changes indicate the concentration of the stimulus in the environment.
REFERENCES:
patent: 3966580 (1976-06-01), Janata et al.
patent: 4151049 (1979-04-01), Janata
patent: 4354913 (1982-10-01), Pungor et al.
patent: 4388166 (1983-06-01), Suzuki et al.
Proc. Nat. Acad. Sci. USA, vol 69, No. 12, pp. 3561-3566, Dec. 1972 "Formation of Bimolecular Membranes from Lipid Monolayers and a Study of Their Electrical Properties," M. Montal and P. Mueller.
Pflugers Arch., 391, 85-100 (1981) "Improved Patch-Clamp Techniques for High Resolution Current Recording from Cells and Cell-Free Membrane Patches," O. P. Hamill, A. Marty, E. Neher, B. Sakmann and F. J. Sigworth pp. 481-496.
Science, 220 717-19 (1983), "Formation in Vitro Sperm Pronuclei and Mitotic Chromosomes Induced by Amphibian Ooplasmic Components" Vitaly Vodyanoy and Randall B. Murphy.
Biophys. J., 47, 93a (1985), "Patch Clamping of Bacteriorhodopsin and its Reconstitution in a Polymerizable Lechithin," Paul Yager.
Ber. Bunsenges, Phys. Chem., 86, 499-504, "Cyanine Dyes as Sensitizers of the Photopolymerization of Diacetylenes in Multilayers," C. Bubeck, B. Tieke, and G. Wegner (1982).
Ber. Bunsenges, Phys. Chem., 86, 495-498 (1982) "Selfsensitization of the Photopolymerization of Diacetylenes Studied in Multilayers," C. Bubeck, B. Tieke, and G. Wegner.
Angew. Chem. Suppl. 869-880 (1982), R. Benz, W. Press and H. Ringsdorf, "Black Lipid Membranes from Polymerizable Lipids".
"Reconstitution of Bacteriorhodopsin in a Polymerizable Lecithin" Paul Yager, submitted to Biochim. Biophys. Acta. Jul. 1985.
Beers Robert F.
Ellis William T.
Guffey Wendell R.
Terapane John F.
United States of America
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