Purification of Q Beta replicase

Details Purification of Q Beta replicase Purification of Q Beta replicase

1994-08-18

1997-02-04

Sayala, Chhaya D.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435220, 435849, 424 945, C12N 912

Patent

active

RE0354430

ABSTRACT:
A process of producing highly pure, Q Beta replicase having a high level of activity is described. The present process allows isolation of Q Beta replicase from recombinant bacteria containing a clone of a phage DNA encoding the 65,000 weight subunit of Q Beta replicase. The present process provides an efficient method for producing pure Q Beta replicase which can readily be scaled up to commercial production levels.

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