Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Attached to antibody or antibody fragment or immunoglobulin;...
Patent
1975-09-09
1976-10-05
Padgett, Benjamin R.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Attached to antibody or antibody fragment or immunoglobulin;...
23230B, 1951035R, 424 12, G01N 3316, A61K 4300, G01T 116
Patent
active
039845320
ABSTRACT:
A method for the quantitative determination of renin activity in blood utilizing the measurement of Angiotensin I by treating the blood with ethylenediaminetetraacetic acid (EDTA) and the plasma with phenylmethyl sulfonylfluoride (PMSF) at a preferred pH to ascertain the full range of concentration where PMSF is effective for accurate and quick determination.
Also, a method for the quantitative determination of renin activity in blood utilizing the measurement of Angiotensin I by incubating the samples after antibody addition at room temperature (23.degree. to 30.degree. C) for 1 to 2 hours and separating the free from the antibody bound species with polyethylene glycol after having treated the blood with ethylenediaminetetraacetic acid (EDTA) and the plasma with phenylmethyl sulfonylfluoride (PMSF) at a preferred pH. PMSF offering enough protection against Angiotensin I destruction at room temperature that in combination with polyethylene glycol at a preferred pH the time required for the radioimmunoassay determination is 1 to 2 hours at room temperature instead of 24 .+-. 2 hours at 4.degree. C which takes with charcoal in the presence of other inhibitors and making the system less succeptible to time dependent errors.
REFERENCES:
patent: 3843775 (1974-10-01), Wolf
patent: 3899298 (1975-08-01), Szczesniak
barrett et al., Clinical Chemistry, vol. 18, No. 11, 1972, pp. 1339-1342.
Goodfriend et al., Journal of Laboratory and Clinical Medicine, vol. 72, No. 4, Oct. 1968, pp. 648-651.
Nucker Christine M.
Padgett Benjamin R.
Saffitz Abraham A.
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