Production of GAD65 in methylotrophic yeast

Details Production of GAD65 in methylotrophic yeast Production of GAD65 in methylotrophic yeast

1996-11-08

1999-10-12

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

43525422, 43525423, C12P 2102, C12N 119

Patent

active

059653892

ABSTRACT:
Methylotrophic yeast are used for high-level expression of GAD65 that makes the production of GAD65 feasible on an industrial scale. A methanol-inducible promoter from, for example, an alcohol oxidase gene, such as Pichia pastoris AOX1, can be used to regulate GAD65 expression. The recombinant GAD65 has high specific activity and retains antigenic characteristics of the native molecule that are essential to immunological assays and therapeutic protocols.

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