Process for obtaining cholesterol esterase from microorganisms

Details Process for obtaining cholesterol esterase from microorganisms Process for obtaining cholesterol esterase from microorganisms

1984-10-22

1986-10-07

Hazel, Blondel

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435874, C12N 918

Patent

active

046159819

ABSTRACT:
The present invention provides a process for obtaining cholesterol esterase from micro-organisms by culturing a micro-organism capable of forming cholesterol esterase in an appropriate nutrient medium in the presence of an inductor and obtaining the enzyme from the culture liquid and/or from the cells, therein the inductor used is a compound of the general formula: ##STR1## in which R and R.sub.1 are alkyl or alkoxy radicals containing 14 to 18 carbon atoms and R or R.sub.1 can also be a hydrogen atom and R.sub.2 is an alkylamino radical containing 2 to 8 carbon atoms, an alkyl-trimethylammonium radical containing 3 to 8 carbon atoms, an alkylpyridine radical containing up to 4 carbon atoms in the alkyl moiety or a radical of the general formula --CH.sub.2 --(CHOH).sub.n --CH.sub.2 OH, in which n is a whole number of from 1 to 4.

REFERENCES:
patent: 4052263 (1977-10-01), Masurekar et al.
patent: 4343903 (1982-08-01), Beaucamp et al.
patent: 4360596 (1982-11-01), Beaucamp et al.

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