Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence
Patent
1996-02-16
1998-04-14
Snay, Jeffrey
Chemistry: analytical and immunological testing
Optical result
With fluorescence or luminescence
2504591, G01N 2164
Patent
active
057390401
ABSTRACT:
The present invention is directed to a device and method for determining the presence of fluorescent molecules, locations thereof, and number of fluorescent molecules in a local area irradiated with excitation light based on the number of fluorescence photons generated per unit period of time by the irradiation of the excitation light counted as a quantized fluorescence intensity of the fluorescence molecules and a distribution of appearance frequencies of the fluorescence photons formed on a two-dimensional image. In accordance with the present invention, a device is provided which comprises a light source for irradiating excitation light to a local area on a flat substrate with the adsorbed fluorescent molecules, and fluorescence detector for detecting fluorescence photons from the fluorescent molecules and for forming a distribution of appearance frequencies of the fluorescence photons per unit period of time on a two-dimensional image corresponding to the local area.
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Hamamatsu Photonics K.K.
Snay Jeffrey
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