Physiological cell separation and method of separating cells usi

Details Physiological cell separation and method of separating cells usi Physiological cell separation and method of separating cells usi

1995-06-05

1998-04-14

Rollins, John W.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435404, 435366, C12N 500

Patent

active

057390339

ABSTRACT:
An aqueous composition, initially present as a two-phase gradient, useful for separation of two cell types, such as islet cells from acinar cells, is disclosed, as well as a method of separating the cells. The composition is comprised of a water soluble, metabolically inert substance which maintains density, osmolarity and pH in the presence of cells, and a physiological cold storage solution comprising viscosity modifiers to increase the viscosity and osmotically active impermeant agents for suppressing cold-induced cellular edema, and also comprising a physiological solution capable of maintaining viable cells. These solutions are present in various combinations in a concentration sufficient to provide a first solution with a density greater than both cell types in their natural state, and less than the second cell type after incubation in said solution, and a second solution having a density less than that of both cell types. The system is allowed to stand or is centrifuged to separate the cells.

REFERENCES:
patent: 4640785 (1987-02-01), Carroll et al.
Raydt. Hoppe-Seyler's Z. Physiol. Chem. vol. 358, pp. 1369-1373, 1977.
D'Allessandro et al. Diabetes vol. 38, pp. 7-9, 1989.
Lake et al. Diabetes. vol. 38, pp. 143-145, 1989.

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