Process for purifying an .alpha.-D-galactosidase isozyme from Co

Details Process for purifying an .alpha.-D-galactosidase isozyme from Co Process for purifying an .alpha.-D-galactosidase isozyme from Co

1992-12-23

1996-04-30

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435814, 435815, C12N 940

Patent

active

055124712

ABSTRACT:
A Coffea canephora-D-galactosidase isozyme is purified by extracting a supernatant from Coffea beans containing the isozyme, extracting tannin from the supernatant, and isolating the isozyme. Preferrably, the supernatant is extracted by exposing the supernatant to insoluble polyvinylpolypyrrolidone in an amount sufficient to remove the tannin from the supernatant by forming hydrogen bonds with the tannin.

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