Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1984-01-31
1990-05-15
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 693, 4351723, 4352523, 435320, 435844, 935 31, 935 38, 935 58, 935 61, 935 12, 935 29, 935 72, C12P 2100, C12N 1500, C12N 700, C12R 116
Patent
active
049257927
ABSTRACT:
Process for the production of a protein correlated with the diphtheric toxin which comprises culturing in a liquid nutrient medium having a concentration of iron ions of from 0.05 .mu.g/ml to 0.5 .mu.g/ml, at a temperature of from 30.degree. C. to 40.degree. C., in a neutral culturing environment and under aerobic conditions, a microorganism belonging to the Corynebacterium diphtheriae genus with two mutant phages encoding the protein correlated with the diphtheric toxin integrated in a nontandem mode in their chromosomes.
REFERENCES:
Rappuoli et al. (Jan. 18, 1983), J. Virology, vol. 45, pp. 524-530.
Uchida et al. (1973), J. Biological Chemistry, vol. 248, pp. 3838-3844.
Groman et al. (1979), Infection and Immunity, vol. 26, pp. 1065-1070.
Uchida, T. et al., 1973, JBC, 248: 3838.
Rappuoli, R. et al., Feb. 1983, J. Virol, 45: 524.
Ishii-Kanei, C. et al., Sep. 1979, Infection and Immunity, 25: 1081.
Groman, N. and K. Judge, Dec. 1979, Infection and Immunity, 26: 1065.
Rappuoli, R. et al., Feb. 1985, Bio/Technology, p. 161.
Uchida et al. (1973), J. Biol. Chem., 248: 3838-3844.
Rappuoli et al. (1983), J. Bact., 153: 1202-1210.
Rappuoli (1983), Appl. Env. Microbiol., 46: 560-564.
Sclavo S.p.A.
Wiseman Thomas G.
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