Process for producing proteins correlated with the diphtheric to

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 693, 4351723, 4352523, 435320, 435844, 935 31, 935 38, 935 58, 935 61, 935 12, 935 29, 935 72, C12P 2100, C12N 1500, C12N 700, C12R 116

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049257927

ABSTRACT:
Process for the production of a protein correlated with the diphtheric toxin which comprises culturing in a liquid nutrient medium having a concentration of iron ions of from 0.05 .mu.g/ml to 0.5 .mu.g/ml, at a temperature of from 30.degree. C. to 40.degree. C., in a neutral culturing environment and under aerobic conditions, a microorganism belonging to the Corynebacterium diphtheriae genus with two mutant phages encoding the protein correlated with the diphtheric toxin integrated in a nontandem mode in their chromosomes.

REFERENCES:
Rappuoli et al. (Jan. 18, 1983), J. Virology, vol. 45, pp. 524-530.
Uchida et al. (1973), J. Biological Chemistry, vol. 248, pp. 3838-3844.
Groman et al. (1979), Infection and Immunity, vol. 26, pp. 1065-1070.
Uchida, T. et al., 1973, JBC, 248: 3838.
Rappuoli, R. et al., Feb. 1983, J. Virol, 45: 524.
Ishii-Kanei, C. et al., Sep. 1979, Infection and Immunity, 25: 1081.
Groman, N. and K. Judge, Dec. 1979, Infection and Immunity, 26: 1065.
Rappuoli, R. et al., Feb. 1985, Bio/Technology, p. 161.
Uchida et al. (1973), J. Biol. Chem., 248: 3838-3844.
Rappuoli et al. (1983), J. Bact., 153: 1202-1210.
Rappuoli (1983), Appl. Env. Microbiol., 46: 560-564.

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