Monoclonal anti-idiotypic antibody, method for production thereo

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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436529, 436535, 436548, 530387, 530413, 530402, 530813, 530808, 530809, 43524027, 935103, 935108, 935110, G01N 33577, G01N 33535

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049257870

ABSTRACT:
A monoclonal anti-idiotypic antibody specific to a human IgG.sub.1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor; a method for the production of the aforementioned monoclonal anti-idiotypic antibody by the steps of immunizing an animal with a human IgG.sub.1 type monoclonal antibody specific to nicotinic acetylcholine receptor, collecting antibody-producing cells from the animal, fusing the collected cells with neoplastic cells, selecting from the product of fusion a hybridoma capable of producing a monoclonal anti-idiotypic antibody specific to the human IgG.sub.1 type monoclonal antibody possessing specificity to nicotinic acetylcholine receptor, propagating the selected hybridoma thereby giving rise to said monoclonal anti-idiotypic antibody, and collecting the produced monoclonal anti-idiotypic antibody; and use of the monoclonal anti-idiotypic antibody as a reagent and as an adsorbent.

REFERENCES:
patent: 4536479 (1985-08-01), Vander-Mallie
Lefvert, A. et al., Eur. J. Immunol, 12(9), 790-792, (Sep. 1982)
Mehraban, F. et al., Eur. J. Biochem., 138(1), 53-61, (Jan. 1984).
Nature, (1983), vol. 301, pp. 611-614, "Naturally Occurring Anti-Idiotypic Antibodies in Myasthenia Gravis Patients", Dwyer et al.
Eur. J. Immunol., (1982), vol. 12, pp. 790-792, "A Monoclonal Anti-Idiotypic Antibody Against Anti-Receptor Antibodies From Myasthenic Sera", Lefvert et al.

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