Method for the purification of lymphokine LK 2

Details Method for the purification of lymphokine LK 2 Method for the purification of lymphokine LK 2

1988-07-21

1991-03-26

Nucker, Christine

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

435 703, 530351, C07K 320

Patent

active

050030485

ABSTRACT:
A monoclonal antibody specific to the lymphokine LK 2, and its production is disclosed. The novel lymphokine LK 2 is a glycoprotein with a molecular weight of 20,000.+-.2,000 daltons; isoelectric point pI, 6.2.+-.0.3; electrophoretic mobility Rf, 0.29.+-.0.02; cytotoxic on L 929 cells; and substantially not growth-inhibitive on KB cells. The lymphokine significantly inhibits the growth of malignant human tumors in vivo. The monoclonal antibody may be of IgM or IgG class, and neutralizes specifically the cytotoxic activity of the lymphokine. Combined use of LK 2 with chemotherapeutic agents such as alkylating agents, metabolic antagonists, antioncotic antibiotics and plant alkaloids enhances greatly the antioncotic effect of the chemotherapeutics.

REFERENCES:
patent: 4276282 (1981-06-01), Sugimoto
patent: 4285929 (1981-08-01), Sugimoto et al.
patent: 4758549 (1988-07-01), Mitsuhashi et al.
Schneider, et al., J. Biol. Chem., vol. 257, pp. 10766-10769, 1982.
Scope, R., In Protein Purification, Springer-Verlag, N.Y., 1982, pp. 132-135, 172-177 and 250-251.
Examiner's Report issued in the Corresponding Australian Patent Application No. 49708/85 along with copies of the references acited in the Examiner's Report.

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