Concatemeric DNA length standards

Details Concatemeric DNA length standards Concatemeric DNA length standards

1989-01-04

1991-07-09

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435219, 435220, 435221, 435222, 4352351, 435259, 435822, 435849, 536 26, 536 27, 536 28, 436 8, C12P 1934, C12N 950, C12N 956, C07H 152

Patent

active

050305662

ABSTRACT:
The use of T7 bacteriophage to produce DNA length standards by enzymatically joining terminally repetitious, blunt-ended DNA has now been demonstrated. It is now possible to precisely control the formation of concatemeric DNAs thereby generating custom-made size-ranges of length standards. Furthermore, the standards thus produced are stable over time providing a highly reproducible and convenient product for the molecular biologist.

REFERENCES:
patent: 4650761 (1987-03-01), Hershberger et al.
patent: 4784952 (1988-11-01), Ludwig et al.
John H. White, "Formation and Processing of Concatemers of Bacteriophage T7 DNA, In Vitro", Harvard Medical School, Harvard University (1987).

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