Diagnostic agents

Details Diagnostic agents Diagnostic agents

1978-04-24

1980-10-07

Gron, Teddy S.

Chemistry: physical processes

Physical processes

Crystallization

210716, 424 3, 424 12, 252408, 435 11, 260112R, C09K 300, G01N 3102, G01N 3106, G01N 3114, G01N 3316

Patent

active

042267137

ABSTRACT:
Diagnostic agents are described for determining high density lipoprotein cholesterol in body fluids such as serum or plasma. One is an enzymatic reagent for quantitative analysis for cholesterol composed of cholesterol oxidase, cholesterol esterase, peroxidase, 4-amino-antipyrine, a phenol or phenol derivative, a surfactant, such as sodium cholate and a polyglycol of molecular weight from about 190 to 1000, buffered to pH 5.5 to 7.8 in water. The other is a single, stable precipitating agent for lipoproteins in body fluids, comprising a bivalent metal salt of a monobasic acid, such as magnesium chloride and a water-soluble polyanion, such as sodium phosphotungstate in water at pH 5 to 8.

REFERENCES:
patent: 3955925 (1976-05-01), Proksch et al.
patent: 4011045 (1977-03-01), Bonderman
patent: 4039285 (1977-08-01), Teipel
patent: 4045176 (1977-08-01), Proksch et al.
patent: 4147606 (1979-04-01), Golias
Burstein, M. et al., J. Lipid Res., vol. 11, pp. 583-595, (1970).
Tietz, N. W., Fundamentals of Clinical Chemistry, W. B. Saunders Company, Philadelphia, Pa. pp. 539-540 (1976).

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