Method and kit to assay for steroid receptor functionality in ca

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Sample mechanical transport means in or for automated...

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424 3, 435 29, 435 34, 435267, 435810, 436175, 436501, 436503, 436504, 436808, 436813, 436817, G01N 33574

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050304178

ABSTRACT:
A method and a test kit for rapidly determining the presence of functional cellular steroid receptors by assaying a tissue sample for nuclear steroid or antisteroid binding is disclosed which comprises treating the tissue with collagenase, incubating the isolated cells with a labelled steroid or a labelled antisteroid capable of complexing said receptors and measuring the bound nuclear radioactivity and the DNA of the isolated cellular nuclei.

REFERENCES:
patent: 4215102 (1980-07-01), Lee
patent: 4232001 (1980-11-01), Jensen et al.
patent: 4423151 (1983-12-01), Baranczuk
patent: 4433056 (1984-02-01), Baranczuk
T. C. Spelsberg et al., in "Clinical Laboratory Molecular Analyses," Grune & Stratton, Inc., (1985), at pp. 189-203.
I. Jung-Testas et al., J. Steroid Biochem., 20, 301, (1984).
R. R. Weichselbaum et al., J. Cell. Physiol., 103, 429, (1980).
D. Huchon, Ann. Endocrinol., 8, 223, (1977), (English Abstract Only).
L. Laine et al., The Lancet, p. 168, (7-23-77).
A. G. Fazekas and J. K. MacFarlane, The Lancet, p. 565, (1982).
R. E. Leake et al., Brit. J. Cancer, 43, 59, (1981).
S. G. Korenman, J. Clin. Endocrinol. Metab., 28, 127, (1968).
K. J. Andersen et al., Anal. Biochem., 83, 703, (1977).
M. L. Graham, II et al., Mayo Clin. Proc., 59, 3, (1984).
P. A. Boyd et al., Biochem., 18, 3685, (1979).
P. A. Boyd-Leinen et al., Endocrinology, 111, 30, (1982).
P. A. Boyd-Leinen et al., J. Biological Chem., 259, 2411, (1984).
A. Geier et al., J. Endocrinol., 80, 281, (1979).
T. Thorsen, J. Steroid Biochem., 10, 661, (1979).
A. G. Fazekas et al., J. Steroid Biochem., 13, 613, (1980).
J. K. MacFarlane et al., Cancer, 45, 2998, (1980).
T. Thorsen and S. K. Stoa, J. Steroid Biochem., 10, 595, (1979).
Holdaway et al., Cancer Res., 52, 479, (1983).
Okret et al., Cancer Res., 38, 3904, (1978).
Duvivier et al., Clinica Chimica Acta, 112, 21, (1981).
K. Burton, Biochem. J., 62, 315, (1956).

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