Nucleic acid sequence determination by multiple mixed oligonucle

Details Nucleic acid sequence determination by multiple mixed oligonucle Nucleic acid sequence determination by multiple mixed oligonucle

1988-10-24

1991-03-26

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435810, 536 27, 935 77, 935 78, 436501, 436808, C12Q 168, C07H 1512, C12N 1500, G01N 33566

Patent

active

050028677

ABSTRACT:
A method is provided for sequencing nucleic acids without the need to separate similarly sized DNAs or RNAs by gel electrophoresis. The method relies on the separate hybridization of multiple mixed oligonucleotide probes to a target sequence. The mixed oligonucleotide probes comprise sequences of fixed and non-fixed bases corresponding to every possible permutation of fixed and non-fixed bases less than or equal to the length of the probes. For each probe, the hybridizations provide the number of times the probe's particular sequence of fixed bases appears in the target sequence. The target sequence is then mathematically reconstructed from this data and a knowledge of the probe sequences.

REFERENCES:
patent: 4865968 (1989-09-01), Orgel et al.

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