Polarographic method for measuring both analyte and oxygen with

Chemistry: electrical and wave energy – Processes and products – Processes of treating materials by wave energy

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20415312, 20415316, 128635, G01N 2748

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active

050303333

ABSTRACT:
Novel polarographic (voltametric) methods for measuring in vivo or in vitro analytes in an animal tissue and oxygen in the sensor enzymatic reaction layer with an electroenzymatic sensor are disclosed. The new and vastly improved methods are directed to generating from the electroenzymatic sensor at a suitable voltage an anodic current derived from an anodically active product generated by enzymatic activity on an analyte for measuring the analyte and a cathodic current derived from reducing the oxygen in the sensor enzymatic reaction layer for measuring the oxygen. Additionally, the method contemplates switching the voltage between anodic and cathodic potentials to generate anodic and cathodic currents or applying the voltage at a fixed rate. Advantageously, the methods of this invention may be utilized with implanted sensors in an animal including a human. The sensors may be implanted in blood or non-blood sites. Finally, the methods of this invention further contemplate analyzing the type of conditions around the implanted sensor site to improve the reliability of the sensor as well as the detection of the analyte by the sensor.

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patent: 4458686 (1984-07-01), Clark, Jr.
patent: 4467811 (1984-08-01), Clark, Sr.

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