Apparatus for quantitatively determining the level of hemoglobin

Details Apparatus for quantitatively determining the level of hemoglobin Apparatus for quantitatively determining the level of hemoglobin

1983-03-31

1985-09-03

Turk, Arnold

Chemical apparatus and process disinfecting, deodorizing, preser

Analyzer, structured indicator, or manipulative laboratory...

Calorimeter

128757, 422 61, 422 68, 422 99, 436 66, 436172, G01N 2175, G01N 3352, G01N 3372

Patent

active

045391807

ABSTRACT:
A method of quantitatively determining the level of hemoglobin in a biological material which includes the steps of preparing a test sample of the biological material, converting the heme portion of the hemoglobin in the test sample to porphyrin, assaying the fluorescence of the converted porphyrin and comparing the fluorescence of the converted porphyrin to a standard. The invention also relates to an apparatus for performing the above method and a sampler device for collecting and preparing a test sample of the biological material, wherein the apparatus includes at least two reaction chambers with one chamber containing oxalic acid and ferrous oxalate for converting the heme portion of hemoglobin in a test sample to porphyrin and the other chamber containing citric acid which is generally non-reactive with the heme portion of hemoglobin to function as a control blank for the hemoglobin determination.

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Wang et al., J. Sci. Food Agric., vol. 30, pp. 1089-1096, (1979).

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