Method for determining the degree of spectral interference in an

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

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356417, 2504581, 2504591, G01N 2164

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active

054595673

ABSTRACT:
A method to determine the degree of spectral interference in an assay containing a test sample in which a graph plot of efficiency versus ratio of a fluorescence label is a known standard. The assay is placed in a fluorimeter and excited with light energy at the excitation wavelength of the fluorescence label. The entire emission spectrum is examined and an actual fluorescence emission intensity value is obtained from the assay which is representative of the activity of the test sample.

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