Cloning of a gene encoding for the growth hormone releasing horm

Details Cloning of a gene encoding for the growth hormone releasing horm Cloning of a gene encoding for the growth hormone releasing horm

1995-05-01

1997-07-01

Walsh, Stephen

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2431, 435 6, 435 912, 435 691, 935 13, C12N 1512

Patent

active

056440464

ABSTRACT:
Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromatography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.

REFERENCES:
Mayo et al., Molecular Endocrinology, 6, 1734-1743, 1992.
Hsiung et al., Neuropeptides, 25, 1-10, 1993.

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