Chemiluminescence method for assaying compounds containing prima

Details Chemiluminescence method for assaying compounds containing prima Chemiluminescence method for assaying compounds containing prima

1986-03-10

1988-07-19

Richman, Barry S.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436 89, 436109, 436 93, 436111, 436128, 436131, 436135, 436172, G01N 2176, G01N 3352, G01N 3368

Patent

active

047585200

ABSTRACT:
A chemiluminescence method for assaying compounds containing primary amino groups using 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole fluorescers is disclosed. The 1-cyano-2-substituted benz(f)- or naphth(f)-isoindole (CBI or CNI) is formed by reacting a compound containing a primary amino group with naphthalene-2-3-dicarboxaldehyde or anthracene-2,3-dicarboxaldehyde in the presence of cyanide ion. The reaction product of hydrogen peroxide with an oxalate ester is then combined with the CBI or CNI to form an analyte which exhibits chemiluminescence. A detector is used to measure the chemiluminescence emission from the chemiluminescence derivatized analytes. Compounds containing primary amino groups which may be assayed according to the present invention include primary amines, amino acids, peptides and catecholamines. The method can also be adapted to the analysis of trace amounts of cyanide ion and hydrogen peroxide.

REFERENCES:
patent: 3511612 (1970-05-01), Kennerly et al.
patent: 3539794 (1970-11-01), Rauhut et al.
patent: 3557233 (1971-01-01), Zweig et al.
patent: 3576987 (1971-05-01), Voight et al.
patent: 3578962 (1971-05-01), Gerber
patent: 3597362 (1971-08-01), Bollyky et al.
patent: 3689391 (1972-09-01), Ullman
patent: 3691085 (1972-09-01), Roberts et al.
patent: 3704231 (1972-11-01), Bollyky
patent: 3704309 (1972-11-01), Maulding
patent: 3718432 (1973-02-01), Roth
patent: 3718599 (1973-02-01), Rauhut
patent: 3729426 (1973-04-01), Zweig et al.
patent: 3775336 (1973-11-01), Kasulin et al.
patent: 3892530 (1975-07-01), Felix et al.
patent: 3933430 (1976-01-01), Hare
patent: 4359323 (1982-11-01), LePage
patent: 4647532 (1987-03-01), Watanabe et al.
Honda et al., J. of Chromatography, vol. 303, pp. 173-176, 1984.
Kobayashi et al., Anal. Biochem., vol, 112, pp. 99-104, 1981.
Honda et al., J. of Chromatography, vol. 316, pp. 501-505, 1984.
Rauhut et al., J. Am. Chem. Soc., vol. 89, pp. 6515-6522, 1967.
Kobayashi et al., Anal. Chem., vol. 52, pp. 424-427, 1980.
Miura et al., Anal. Biochem., vol. 137, pp. 432-437, 1984.
Ishida et al., J. of Chromatography, vol. 204, pp. 143-148, 1981.
Gardner et al., Anal. Biochem, vol. 101, pp. 61-65, 1980.
Allison et al., Anal. Chem., vol. 56, pp. 1089-1096, 1984.

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